Nano pore gene sequencing reads longer, making it easier to cover complex and structurally variable regions. The obtained long fragments can have more overlapping areas, which is more conducive to assembling and obtaining the correct base sequence. It can also obtain accurate sequences such as repetitive sequences that are difficult to assemble by short fragment sequencing, making it easier to assemble and obtain more complete microbial genomes or plasmids, and to conduct detailed composition analysis of functional elements such as drug resistance and virulence.

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The use of long segment sequencing technology to analyze microbial genomes can simultaneously identify single nucleotide polymorphism sites (SNPs), Single Nucleotide Polymorphism）、 By inserting missing sites (InDel, Insertion/Deletion) and structural variations, comprehensive and integrated information on microbial genome mutations can be obtained, which is more conducive to conducting research on species evolution, population characteristics, selection pressure, and horizontal gene transfer.

When using amplicon sequencing to study environmental microbial diversity, long fragment sequencing can cover the entire conserved and variable region sequences (such as 16S rRNA, 18S rRNA, ITS, etc.), which is more conducive to accurate identification of microbial species and species level classification and identification, thus more accurately studying the relationship between species composition and environment.