On November 3rd, the "Witness the 'Core' Birth, Co-Appreciate the 'Pore' Future | New-Generation Gene Technology Innovation and Development Industry Forum" was held. Centering on the theme of "Collaborative Innovation of Solid-State Nanopore Materials, Devices, and Algorithms," experts from institutions including Peking University, Southeast University, Institute of Biotechnology of the Chinese Academy of Sciences, and Nanchang University gathered to conduct in-depth discussions on technical bottlenecks and clinical transformation. The forum was hosted by Liu Quanjun, and five experts—Zhao Qing, He Qinghua, Xi Dongmei, Zhao Xiangwei, and Jiang Huifeng—offered suggestions and insights from the perspectives of materials science, biology, clinical practice, algorithms, and synthetic biology respectively.

Zhao Qing pointed out that solid-state nanopore sequencing needs to improve both spatial and temporal resolution simultaneously. He suggested optimizing material purity and interface engineering in ultra-thin film structures of 5–10 nm to reduce low-frequency noise.

He Qinghua believes that solid-state nanopores are expected to achieve whole-cell multi-target detection of pathogenic microorganisms, but it is necessary to break through the bottlenecks of sample pretreatment and signal specificity.

Xi Dongmei emphasized that interfering molecules in clinical samples are complex, and surface modification and specific recognition strategies should be adopted to enhance the anti-interference ability of disease marker detection.

Zhao Xiangwei proposed that aiming at the characteristics of low-dimensional data from solid-state nanopores, machine learning-based feature extraction and dimension enhancement algorithms should be developed to improve base-calling accuracy.

Jiang Huifeng stated that current DNA synthesis efficiency relies on traditional methods such as gel electrophoresis, and solid-state nanopores, with their potential for high-resolution conformational analysis, are expected to become a new type of evaluation tool.

Experts unanimously agree that solid-state nanopores outperform biological nanopores in terms of stability, integration, and scalability. However, they still need to make breakthroughs in single-base accuracy, molecular manipulation, and system integration. In the future, it is necessary to strengthen interdisciplinary collaboration, accelerate the translation of the technology from the laboratory to practical scenarios such as precision medicine and synthetic biology, and support the leapfrog development of domestic sequencing technology.